BYRSONIMA INTERMEDIA PDF

Published15 Jun Abstract Bioassay-guided fractionation of the chloroform extract of Byrsonima fagifolia leaves led to the isolation of active antitubercular compounds alkane dotriacontane Minimal Inhibitory Concentration—MIC, The antimycobacterial activity was determined by the Microplate Alamar Blue Assay MABA and the structures of promising compounds were determined by spectroscopic analysis. This investigation constitutes the first report of a chemical and antitubercular study of apolar compounds from B. Introduction Mycobacterium tuberculosis, the agent of tuberculosis, is responsible for high mortality worldwide, killing roughly 1. The situation with multidrug resistant MDR tuberculosis TB today worries the health authorities of the whole world, mainly in the developing countries, where the situation is more severe. The appearance of resistant strains to the medicines now in use makes urgent the search for new synthetic or natural tuberculostatic drugs.

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Published15 Jun Abstract Bioassay-guided fractionation of the chloroform extract of Byrsonima fagifolia leaves led to the isolation of active antitubercular compounds alkane dotriacontane Minimal Inhibitory Concentration—MIC, The antimycobacterial activity was determined by the Microplate Alamar Blue Assay MABA and the structures of promising compounds were determined by spectroscopic analysis. This investigation constitutes the first report of a chemical and antitubercular study of apolar compounds from B.

Introduction Mycobacterium tuberculosis, the agent of tuberculosis, is responsible for high mortality worldwide, killing roughly 1. The situation with multidrug resistant MDR tuberculosis TB today worries the health authorities of the whole world, mainly in the developing countries, where the situation is more severe.

The appearance of resistant strains to the medicines now in use makes urgent the search for new synthetic or natural tuberculostatic drugs. There are several reasons that justify the need to search for new drugs for TB, for example, improvement of current treatment by shortening its duration, to get efficient treatment for MDR TB and to eradicate the latent infection.

So, the development of new drugs for shortening the duration of the treatment and to fight against multidrug resistant tuberculosis strains is urgent [ 2 ]. An approach to the search for new drugs is to look in nature, mainly for the extremely rich and varied flora of the tropical areas. In this search, information obtained from folk knowledge and traditional medicine of different cultures can be valuable.

This trend in associating popular or alternative medicine with pharmaceutical research has been growing for some years [ 3 ]. Byrsonima fagifolia Niedenzu IK is a member of the Malpighiaceae family and is a native species from the Brazilian Cerrado savannah-like vegetation.

The Cerrado is considered the most extensive woodland-savannah in South America and contains over species of higher plants [ 4 ], some of then with antimicrobial activities [ 5 ].

In Brazilian folk medicine, the leaves of B. Previously, we also have reported the antiulcer activity of B. Despite the popular use of B. Plants extracts are attractive sources of new drugs, and bioassay-guided fractionation is the state-of-art process to identify the active compounds contained in crude natural products.

Methods 2. Plant Material The fresh leaves of B. A voucher specimen was deposited in the herbarium of the same university under the number Extraction and Isolation The air-dried and powdered leaves 2. A portion of the chloroform extract The column was eluated sequentially with hexane, then dichloromethane and finally methanol.

Evaporation of the solvents yielded the dry eluates from hexane 0. The fraction eluated by hexane 0. Analogously, the other two fractions eluated with methanol and dichloromethane were fractionated on a similar column Hydrogen was used as the carrier gas 60 kPa , and the injection split ratio was 1 : The fraction eluated by hexane from the silica gel column of the B. The NMR spectra data obtained were compared with those reported in the literature [ 9 ].

Similarly, other triterpenes may be identified in mixtures, without previous separation [ 10 ]. Suspensions were prepared and their turbidities matched to a McFarland no. Samples were set up in triplicate. For standard test, the MIC value of isoniazid was determined each time. The acceptable MIC of Isoniazid ranged from 0.

Results The fractions originating from the initial chromatography of the extract in chloroform of B. The triterpene bassic acid mg was obtained from 2. The structures of the compounds are shown in Figure 1. Figure 1 Compounds identified from the chloroform extract of Byrsonima fagifolia. MIC of The MIC of Isoniazid, used as a positive control drug, was 0.

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J Ethnopharmacol. Epub Jan Byrsonima intermedia A. Author information: 1 Univ. Malpighiaceae , a medicinal species commonly found in the Brazilian Cerrado, can be used against gastroduodenal disorders, such as gastric ulcers and diarrhea. The gastroprotective effect of MBI was assessed by analysing the volume of gastric juice, pH, total acidity, mucus, NO, sulfhydryl compound, vanilloid receptor, glutathione GSH levels, and myeloperoxidase MPO activity in the gastric and duodenal mucosa.

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Byrsonima intermedia

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